### What is the largest cell culture flask?
The largest cell culture flask available in the market is commonly known as the "T225" flask. This type of flask has a total volume of 225 cm² and is designed for culturing a large number of cells in a single vessel.
### How is the T225 flask used in cell culture?
The T225 flask is mainly used for large-scale cell culture experiments in research labs and biopharmaceutical companies. Here is a step-by-step guide on how to use the T225 flask in cell culture:
1. Preparation: Before using the T225 flask, ensure that the flask is sterile and free from any contaminants. This can be achieved by autoclaving or using sterile filtration techniques.
2. Cell seeding: Add the appropriate amount of cell culture media into the T225 flask, making sure to cover the entire surface area. Then, gently add the desired number of cells into the flask.
3. Incubation: Place the T225 flask in a cell culture incubator set at the optimal temperature and CO2 levels for the specific cell type being cultured. This promotes cell growth and proliferation.
4. Media change: Regularly change the cell culture media in the T225 flask to provide the cells with fresh nutrients and remove waste products. This helps maintain cell health and viability.
5. Sub-culturing: When the cells in the T225 flask reach confluence, they need to be sub-cultured or passaged into new flasks to prevent overcrowding and maintain optimal growth conditions.
6. Harvesting: Once the cells have grown to the desired density or reached a specific experimental endpoint, they can be harvested from the T225 flask for downstream applications such as protein production or cell analysis.
By following these simple steps, researchers can effectively utilize the T225 flask for large-scale cell culture experiments, making it an essential tool in the field of cell biology and biotechnology.
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